Nhanganyaya yeElisa Plate

ELISA PLATE: MuEnzyme Yakabatanidzwa Immunosorbent Assay (ELISA), kuchena, kusungirirwa uye chiyero chemaantigen, ma antibodies, akanyorwa ma antibodies kana maantigen anobatanidzwa mukugadzirisa kwekudzivirira; buffer type, concentration uye Mamiriro ezvinhu akadai sesimba reionic, pH kukosha, kuita tembiricha uye nguva inoita basa rakakosha. Mukuwedzera, pamusoro peyakasimba-phase polystyrene (Polystyrene) semutakuri anoitawo basa rinokosha mukushambadza kwemaantigen, antibodies kana antigen-antibody complexes.

Maantigen, masoja ekudzivirira chirwere uye mamwe mabiomolecules anoiswa kune inotakura pamusoro kuburikidza nenzira dzakasiyana siyana, kusanganisira passive adsorption kuburikidza nehydrophobic bond, hydrophobic/ionic bond, covalent bonding kuburikidza nekuunzwa kwemamwe mapoka anoshanda seamino nekabhoni mapoka, uye kuburikidza nekugadziriswa kwepasi. . Hydrophilic bonding mushure mekuita zvepabonde.

TheElisa Plateinogona kukamurwa kuita 48-tsime uye 96-zvakanaka maererano nehuwandu hwemakomba. Iyo inowanzo shandiswa ndeye 96-tsime, iyo inofanirwa kusarudzwa zvinoenderana neyako microplate muverengi.

Mukuwedzera, kune zvinobviswa uye zvisingatauriki. Kune avo vasina-detachable, slats pabhodhi rose akabatanidzwa pamwe chete. Zvadaro, kune zvinogona kubviswa, slats pabhodhi inoparadzaniswa, uye mapuranga akaparadzaniswa Pane 12-hole uye 8-hole strips. Kazhinji, mahwendefa ane enzyme-akanyorwa anowanzo kushandiswa mazuva ano. Kana wakambotenga mahwendefa akadaro, unogona kungotenga mamwe enzyme-yakanyorwa mitsetse izvozvi.

Kunyange zvazvo ma microplates akaitwa nevagadziri vakasiyana-siyana anotaridzika akafanana zvachose, mamwe madiki madiki achave akasiyana, akadai sechimiro, nezvimwe. Izvi zvinonyanya nekuti zvinoda kushandiswa nevaverengi vakasiyana ve microplate. Naizvozvo, paunenge uchishandisa Paunenge uchisarudza kutenga microplate muverengi, iwe unofanirwawo kufunga kuti microplate muverengi wako anotaridzika sei. Asi kazhinji ivo vakagadziridzwa, zvimwe chete zvichave zvakasiyana. Nekuti zvinhu zve enzyme plate zvinowanzo polystyrene (PS), uye polystyrene haina kugadzikana kwemakemikari uye inogona kunyungudutswa nemhando dzakasiyana dzeorganic solvents (sezvinonhuhwirira hydrocarbons, halogenated hydrocarbons, nezvimwewo), uye ichasvibiswa nemaasidhi akasimba. uye alkalis. , isingamiri kugirizi, uye iri nyore kushandura ruvara mushure mekuvhenekwa nechiedza che ultraviolet, saka iva nechokwadi chekuteerera kune izvi paunenge uchishandisaElisa Plate.