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Ndeapi mabasa eELISA kit?
2022-12-23
ELISA kit yakavakirwa pachikamu chakasimba cheantigen kana antibody uye enzyme yakanyorwa yeantigen kana antibody. Iyo antigen kana antibody yakasungirirwa pamusoro peiyo yakasimba inotakura ichiri kuchengetedza yayo immunological chiitiko, uye iyo enzyme yakanyorwa antigen kana antibody inochengeta zvese zvayakaita immunological uye enzyme chiitiko. Panguva yesarudzo, mufananidzo uri mukuedzwa (umo panopimwa masoja ekudzivirira chirwere kana antigen) unopindirana neantigen kana masoja ekudzivirira chirwere ari pamusoro pemutakuri akasimba. Iyo antigen-antibody complex yakaumbwa pane yakasimba mutakuri inoparadzaniswa kubva kune zvimwe zvinhu zviri mumvura kuburikidza nekugeza.
Enzyme-labeled antigens kana masoja ekudzivirira chirwere anowedzerwa, ayo anosunga kune yakasimba mutakuri nekuita. Panguva ino, huwandu hwe enzyme muchikamu chakasimba chiri muchiyero chehuwandu hwezvinhu mumuenzaniso. Mushure mekuwedzera iyo substrate yeiyo enzyme reaction, iyo substrate inogadziriswa neiyo enzyme kuti ive zvigadzirwa zvemavara. Kuwanda kwechigadzirwa kwakabatana zvakananga nehuwandu hwezvinhu zvakaedzwa mumuenzaniso, saka kuongororwa kwehutano kana kuwanda kunogona kuitwa maererano nekudzika kwemuvara.
Iyo yakanyanya catalytic kunyatsoshanda kwema enzymes nenzira isina kunanga inosimudza mhedzisiro yemhinduro yekudzivirira, ichiita kuti assay inzwe zvakanyanya. ELISA inogona kushandiswa kuona maantigen, asi zvakare inogona kushandiswa kuona masoja ekudzivirira chirwere.
Nheyo dzekutanga dzeELISA kit
Inoshandisa chaiyo maitiro eantigen uye antibody kubatanidza chinhu kune enzyme, uyezve inoburitsa ruvara rwekuita pakati pe enzyme uye substrate yekutsunga kwehuwandu. Chinhu chekuyerwa chinogona kuva antibody kana antigen.
Pane matatu reagents anodiwa mune iyi nzira yekutsunga:
â Solid phase antigen kana antibody (immune adsorbent)
â¡ Enzyme yakanyorwa kuti antigen kana antibody (marker)
⢠substrate ye enzyme chiito (ruvara rwekusimudzira mumiririri)
Muchiyero, antigen (antibody) inotanga kusungwa kune yakasimba mutakuri, asi ichiri kuchengetedza maitiro ayo ekudzivirira muviri, uye ipapo conjugate (marker) ye antibody (antigen) uye enzyme inowedzerwa, iyo inoramba ichichengetedza maitiro ayo ekutanga ekudzivirira uye enzyme. chiitiko. Kana iyo conjugate inopindirana neantigen (antibody) pane yakasimba mutakuri, iyo inoenderana substrate ye enzyme inowedzerwa. Kureva, catalytic hydrolysis kana REDOX maitiro uye ruvara.
Enzyme-labeled antigens kana masoja ekudzivirira chirwere anowedzerwa, ayo anosunga kune yakasimba mutakuri nekuita. Panguva ino, huwandu hwe enzyme muchikamu chakasimba chiri muchiyero chehuwandu hwezvinhu mumuenzaniso. Mushure mekuwedzera iyo substrate yeiyo enzyme reaction, iyo substrate inogadziriswa neiyo enzyme kuti ive zvigadzirwa zvemavara. Kuwanda kwechigadzirwa kwakabatana zvakananga nehuwandu hwezvinhu zvakaedzwa mumuenzaniso, saka kuongororwa kwehutano kana kuwanda kunogona kuitwa maererano nekudzika kwemuvara.
Iyo yakanyanya catalytic kunyatsoshanda kwema enzymes nenzira isina kunanga inosimudza mhedzisiro yemhinduro yekudzivirira, ichiita kuti assay inzwe zvakanyanya. ELISA inogona kushandiswa kuona maantigen, asi zvakare inogona kushandiswa kuona masoja ekudzivirira chirwere.
Nheyo dzekutanga dzeELISA kit
Inoshandisa chaiyo maitiro eantigen uye antibody kubatanidza chinhu kune enzyme, uyezve inoburitsa ruvara rwekuita pakati pe enzyme uye substrate yekutsunga kwehuwandu. Chinhu chekuyerwa chinogona kuva antibody kana antigen.
Pane matatu reagents anodiwa mune iyi nzira yekutsunga:
â Solid phase antigen kana antibody (immune adsorbent)
â¡ Enzyme yakanyorwa kuti antigen kana antibody (marker)
⢠substrate ye enzyme chiito (ruvara rwekusimudzira mumiririri)
Muchiyero, antigen (antibody) inotanga kusungwa kune yakasimba mutakuri, asi ichiri kuchengetedza maitiro ayo ekudzivirira muviri, uye ipapo conjugate (marker) ye antibody (antigen) uye enzyme inowedzerwa, iyo inoramba ichichengetedza maitiro ayo ekutanga ekudzivirira uye enzyme. chiitiko. Kana iyo conjugate inopindirana neantigen (antibody) pane yakasimba mutakuri, iyo inoenderana substrate ye enzyme inowedzerwa. Kureva, catalytic hydrolysis kana REDOX maitiro uye ruvara.
Mumvuri weruvara rwaunogadzira unoenzana nehuwandu hweantigen (antibody) huchayerwa. Ichi chigadzirwa chine ruvara chinogona kutariswa neziso rakashama, optical microscope, electron microscope, inogona kuyerwa zvakare ne spectrophotometer (enzyme label instrument). Iyo nzira iri nyore, yakanakira, inokurumidza uye yakananga.
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