Zvakajairwa Zvishandiso zveELISA Plates

Sechishandiso chekuyedza, iyo yakakosha chimiro cheELISA ndironhevedzano yemamicroplates ane yakasimba chikamu zvinhu (senge mapuroteni nemasoja ekudzivirira chirwere). Mukushandiswa kweiyo ELISA ndiro, sampu ichayedzwa ichaita neayo enzyme-yakanyorwa molecule, uyezve inooneka shanduko yeruvara ichagadzirwa nekuwedzera matrix substrate, uye zvirimo kana chiitiko chechinangwa chemorekuru chinozoverengerwa. kana kuongororwa nekuona chiratidzo chekunyura kana chefluorescence. Aya anotevera akajairika mashandisirwo eELISA mahwendefa munzvimbo dzakasiyana:

1. Protein quantitative analysis: ELISA maplates anogona kushandiswa kuyera kusungwa uye kuita kweprotein mubiological samples seserum uye cell supernatants, zvichipa maturusi ane simba ekuona mabundu markers, hepatitis virus antibodies, myocardial injury markers, etc., uye kubatsira vanachiremba mukukurumidza kuongororwa uye kuongorora zvirwere.

2. Cytokine monitoring: Mukutsvaga kweimmunology,ELISA maplatesinogona kuyera cytokine mazinga musero tsika supernatants kana zvinwiwa zvetishu, izvo zvinobatsira kunzwisisa maitiro ebhayoloji akadai semhinduro dzezvirwere zvemuviri uye mhinduro dzekuzvimba, uye zvine kukosha kukuru pakuvandudzwa kwemishonga mitsva nemishonga.

3. Tsvakurudzo yeNucleic acid: Kuburikidza nemahwendefa eELISA, masayendisiti anogona kuona uye kuongorora zviri mukati uye basa reDNA kana RNA, kupa tsigiro yedata yekutsvakurudza kwema molecular biology senge gene expression uye gene regulation, uye inosimudzira kusimudzira minda senge gene therapy. uye gene editing.

4. Tsvakurudzo yebasa reEnzyme: ELISA mahwendefa anogona kunyatsoyera basa re enzyme, anobatsira vatsvakurudzi kunzwisisa basa uye hurongwa hwekugadzirisa ma enzymes mune zvipenyu, uye kupa zvinyorwa zvakakosha zvekutsvakurudza mune enzyme engineering, metabolic engineering nedzimwe nzvimbo.

5. Intermolecular interaction research:ELISA maplatesinogona kushandiswa kwete chete kuyera zviri mukati mamorekuru, asiwo kudzidza kudyidzana pakati pemamorekuru. Nekubatanidza matekinoroji akadai seyepamusoro plasmon resonance uye fluorescence resonance simba rekufambisa, iyo yekusunga uye yekuparadzanisa maitiro pakati pemamorekuru inogona kutariswa munguva chaiyo, ichipa maonero matsva uye nzira dzekugadzira zvinodhaka, kupindirana kweprotein uye kumwe kutsvagisa.