Sei PCR inoshandiswa kazhinji inogadzirwa nePP?

"Sezvatinoziva tese, PCR inzira yekutanga yekuyedza mumarabhoritari ebiochemical." Migumisiro yekuedza inogara isingagutsikani, iyo inogona kunge yakakonzerwa nekusvibiswa kuduku kwePCR plastiki consumables, kana kupindira kwekuedza kunokonzerwa nekuiswa kweinhibitors. Pane chimwe chikonzero chakakosha: Kusarudzika kusarudzwa kwezvinhu zvinodyiwa kuchavawo nekukanganisa kukuru pamhedzisiro yekuyedza.

Pane zvikonzero zvakawanda zvinokanganisa mhedzisiro yePCR kuyedza: kazhinji kune anotevera marudzi manomwe.

1. Maprimers: Maprimers ndiwo kiyi yemaitiro chaiwo ePCR, uye iyo chaiyo yePCR zvigadzirwa zvinoenderana nehuwandu hwekuenderana pakati pekutanga uye template DNA;

2. Enzyme uye kuisa kwayo;

3. Hunhu uye kusungirirwa kwe dNTP;

4. Template (target gene) nucleic acid;

5. Mg2+ concentration;

6. Kuiswa kwekushisa uye nguva;

7. Nhamba yezvikamu;

8. Equipment, consumables, etc.

Pakati pezvakawanda zvinofurira zvinhu, zvinodyiwa ndezvimwe zvakakosha uye zviri nyore kufuratirwa zvinhu.

Kune marudzi akawanda ePCR inoshandiswa: 8-tubes, low-volume tubes, standard tubes, non-skirted, semi-skirted, full-skirted, uye mutsara wePCR uye qPCR mahwendefa. Zvakaoma kwazvo kusarudza, uye kune akawanda Common matambudziko, ngatitarisei matambudziko anosarudzwa nemunhu wesePCR inoshandiswa, uye sei kuzvipedza?

Sei vachidaroPCR inoshandiswakazhinji yakagadzirwa nePP?

Mhinduro: PCR/qPCR zvinodyiwa zvinowanzogadzirwa nepolypropylene (PP), nekuti ndeye biologically inert material, pamusoro haisi nyore kuomerera kune biomolecules, uye ine yakanaka makemikari kuramba uye tembiricha kushivirira (inogona autoclaved pa 121 madhigirii) mabhakitiriya. uye inogonawo kumira kushanduka kwekushisa panguva yekupisa bhasikoro). Zvinhu izvi zvinowanzosangana zvakananga nemareagents kana samples, saka zvigadzirwa zvemhando yepamusoro uye maitiro akanaka ekugadzirisa zvinoda kusarudzwa panguva yekugadzira nekugadzirira maitiro.