Shamwari dziri murabhoritari dzinowanzovhiringika nemusiyano uri pakati pePCR chubhu, EP chubhu, uye masere-chubhu machubhu? Nhasi ndichasuma kusiyana uye hunhu hwevatatu ava

Shamwari dziri murabhoritari dzinowanzovhiringidzika nekusiyana pakatiPCR chubhus, EP machubhu, uye masere-chubhu machubhu? Nhasi ndichasuma kusiyana uye hunhu hwevatatu ava

1. PCR chubhu

PCR chubhus zvinowanzoshandiswa mukuedza kwebhayoloji. Semuyenzaniso, machubhu eCotaus®PCR anonyanya kushandiswa kupa midziyo yePCR (polymerase chain reaction) kuyedza, iyo inogona kuiswa mukuchinja, kutevedzana, methylation, molecular cloning, gene expression, Genotyping, mushonga, forensic sainzi uye zvimwe minda. Iyo yakajairika PCR chubhu inoumbwa nechubhu muviri uye chivharo, uye iyo chubhu muviri uye chivharo zvakabatanidzwa pamwechete.

Yekutanga PCR chiridzwa chaive chisina chivharo chinopisa. Munguva yePCR maitiro, mvura iri pazasi pechubhu yaizobuda ichienda kumusoro. Kavha yeconvex (kureva kuti, pamusoro pedenga) yakagadzirirwa kufambisa kubuda kwemvura kuti ikome uye kuyerera ichidzika. Nekudaro, chiridzwa chazvino chePCR chiri chirudzi chinopisa chekuvhara. Tembiricha iri pamusoro pechivharo chePCR yakakwira uye tembiricha iri pasi yakaderera. Mvura iri pazasi haisi nyore kunyungudika kusvika kumusoro, saka mazhinji acho anoshandisa zvivharo zvakapfava.

2. EP chubhu

Nekuti iyo centrifuge chubhu yakatanga kugadzirwa uye kuburitswa naEppendorf, inonzi zvakare EP chubhu.

Musiyano mukuru pakatiPCR chubhus uye microcentrifuge machubhu ndeyekuti microcentrifuge chubhu inowanzove yakakora chubhu madziro kuti ive nechokwadi che centrifugation zvinodiwa, ukuwo.PCR chubhus ane akatetepa chubhu madziro kuti ave nechokwadi chekufambisa kupisa kumhanya uye kufanana. Nokudaro, iyo miviri haigoni kusanganiswa mumashandisirwo anoshanda, nokuti machubhu akaonda ePCR anogona kuputika nekuda kwekusakwanisa kutsungirira masimba makuru e centrifugal; zvakafanana, akakora microcentrifuge machubhu anozokanganisa mhedzisiro yePCR nekuda kwekunonoka kwekutamisa kupisa uye kusaenzana kwekutamisa kupisa.

3.machubhu masere

Nekuda kwekuremerwa kwebasa mukuyedzwa kwebatch uye kusashanda zvakanaka kwechubhu imwe chete, machubhu masere mumitsara akagadzirwa.Cotaus®PCR 8-strip chubhu inogadzirwa kubva kunze kwenyika polypropylene, uye chubhu chivharo chinofananidzwa nemuviri wechubhu, ine yakanakisa yekuisa chisimbiso kuita. Panguva imwecheteyo, ine kuchinjika kwakasimba uye inogona kusangana nezvinangwa zvakasiyana zvekuyedza.