Ungasarudza sei PCR/qPCR zvinodyiwa?

PCR inzira inonzwisisika uye inoshanda yekusimudzira kopi imwe chete yechinangwa cheDNA kutevedzana kumamiriyoni emakopi munguva pfupi. Naizvozvo, mapurasitiki ekushandisa ePCR maitiro anofanirwa kunge asina zvinosvibisa uye inhibitors, nepo aine mhando yepamusoro iyo inogona kuvimbisa yakanakisa PCR mhedzisiro. PCR mapurasitiki ekushandisa anowanikwa muhukuru hwakasiyana uye mafomati, uye kuziva hunhu hwakakodzera hwezvigadzirwa zvinokubatsira mukusarudza mapurasitiki akakodzera ekushandisa zvakakwana PCR uye qPCR data.

Kuumbwa uye Hunhu hwePCR zvinoshandiswa

1.Zvinhu
PCR zvinoshandiswa zvinowanzo gadzirwa ne polypropylene, iyo inert inokwana kumira nekukurumidza kuchinja kwekushisa panguva yekupisa bhasikoro uye kuderedza kutorwa kwezvinhu zvinogadzirisa zvinhu kuti ive nechokwadi chemhedzisiro yePCR. zvekurapa-giredhi, zvemhando yepamusoro polypropylene mbishi zvinhu zvinofanirwa kushandiswa panguva yekugadzira uye kugadzirwa muKirasi 100,000 yekuchenesa imba. Chigadzirwa chacho chinofanira kunge chisina nuclease uye kusvibiswa kweDNA kudzivirira kukanganisa mhedzisiro yeDNA amplification kuyedza.

2.Color
PCR mahwendefauyePCR machubhuanowanzo kuwanikwa mune transparent and white.

• Iyo yunifomu yemadziro ukobvu dhizaini ichapa inopindirana kupisa kutamisa kune inopindura samples.

• Yakakwira optical permeability kuti ive nechokwadi chepamusoro fluorescence chiratidzo chekufambisa uye kushoma kukanganisa.

• Mukuedza kweqPCR, gomba jena rakadzivirira kudzokororwa kwechiratidzo chefluorescence uye kutorwa kwayo nemodule yekudziya.

3.Format
Iyo PCR Plate "skirt" yakatenderedza bhodhi. Iyo siketi inopa kugadzikana kuri nani kweiyo pipetting maitiro kana iyo reaction system inovakwa, uye inopa zvirinani simba remuchina panguva yeotomatiki mekiniki kurapwa. PCR ndiro inogona kukamurwa pasina siketi, hafu siketi uye siketi yakazara.

• Iyo isiri-skirted PCR ndiro inoshaikwa yakatenderedza ndiro, uye iyi fomu yekupindura ndiro inogona kuchinjirwa kune yakawanda PCR chiridzwa uye chaiyo-nguva PCR chiridzwa modules, asi kwete yeautomated application.

• Iyo semi-skirted PCR ndiro ine mupendero mupfupi kumucheto kweplate, ichipa rutsigiro rwakakwana panguva yepipetting uye mechanical simba rekubata robhoti.

• Iyo yakazara-skirted PCR ndiro ine mupendero unovhara kureba kweplate. Iyi fomu yeplate yakakodzera kune otomatiki mashandiro, ayo anogona kuve akachengeteka uye akadzikama kugadzirisa. Iyo yakazara siketi zvakare inokwidziridza simba remagetsi, ichiita kuti ive yakakodzera kushandiswa nemarobhoti mune otomatiki kufambiswa kwebasa.

PCR chubhu inowanikwa mune imwechete uye 8-mitsetse chubhu, iyo inonyanya kukodzera kune yakaderera kusvika yepakati throughput PCR/qPCR kuyedza. Iyo yakafuratira kavha yakagadzirirwa kufambisa kunyora, uye iyo yakanyanya kutendeseka kufambisa kweiyo fluorescence chiratidzo inogona kuitwa zvirinani neqPCR.

• Iyo imwechete chubhu inopa kuchinjika kuseta iyo chaiyo nhamba yemaitiro. Kune mavhoriyamu makuru ekuita, chubhu imwe muhukuru hwe 0.5 mL inowanikwa.
• Iyo 8-strips chubhu ine macaps inovhura nekuvhara sampuli machubhu yakazvimiririra kudzivirira sampuli.

4.kuisa chisimbiso
Iyo chubhu yekuvharira uye yekuisa chisimbiso firimu inofanirwa kuvhara zvachose chubhu nendiro kudzivirira kubuda kwemuenzaniso panguva yekupisa. Chisimbiso chakasimba chinogona kuwanikwa nekushandisa firimu scraper uye mudziyo wekudhinda.

• Iyo PCR ndiro matsime ane yakasimudzwa mupendero wakaipoteredza. Iyi dhizaini inobatsira kuvhara ndiro ine chisimbiso firimu kudzivirira kubuda.
• Alphanumeric markings paPCR plate ichabatsira kuona matsime ega ega uye nzvimbo dzemasamples anoenderana. Mabhii ane mabulged anowanzo kudhindwa muchena kana nhema, uye kune otomatiki maapplication, kunyora mabhii kunonyanya kubatsira pakuisa chisimbiso chekunze kwendiro.

5.Flux application

Iyo yekuyedza flux yePCR / qPCR assays inogona kuona kuti ndeipi mhando yezvigadzirwa zvepurasitiki zvinofanirwa kushandiswa pakurapa kwakanyanya. Kune yakaderera-ku-yepakati-yepakati throughput application, machubhu anowanzo kuve akakodzera, nepo mahwendefa ari kunyanya kudiwa kune yepakati-kusvika-yepamusoro throughput kuyedza. Plates akagadzirirwawo kufunga nezve kuchinjika kwe flux, iyo inogona kukamurwa kuita tambo imwe chete.

Mukupedzisa, sechikamu chakakosha chePCR system yekuvaka, zvekushandisa zvepurasitiki zvakakosha pakubudirira kwekuyedza uye kuunganidza data, kunyanya mune yepakati-kusvika-yepamusoro throughput workflow applications.

Semuchina wekuChina anotengesera mapurasitiki ekushandisa, Cotaus inopa matipi epipette, nucleic acid, kuongororwa kweprotein, sero tsika, chengetedzo yemuenzaniso, kuvhara, chromatography, nezvimwe.

Dzvanya pane zita rechigadzirwa kuti uone PCR zvinodyiwa zvigadzirwa.

PCR Tube ;PCR Plate